Journal: bioRxiv

Article Title: Zika and Dengue Viruses Differentially Modulate Host mRNA Processing Factors Defining Its Virulence

doi: 10.1101/2024.10.18.619084

Figure Lengend Snippet: (A) Heatmap of normalized RNA-seq Z-score data for mock versus ZIKV-infected (left) and DENV-infected (right) Vero samples at 1-, 3-, and 5-DPI and long-term infected samples. (B) A comparison of normalized RNA-seq reads represented in reads per million for UPF1 and visualized in IGV. Comparisons shown for mock, ZIKV-, and DENV-infected Vero samples at 1-, 3-, 5-DPI, and long-term infected samples. (C) Western blot analysis of anti-UPF1, anti-UPF2, and anti-alpha tubulin or anti-GAPDH as housekeeping controls for Vero acute ZIKV and DENV infections at 1-, 3-DPI and long-term infections (LTI). (D) Immunocytochemistry staining of Vero mock, ZIKV, and DENV at 1- and 3-DPI. Antibodies used were anti-UPF1 (in green), anti-4G2 (anti-flavivirus, in red), and DAPI (in blue).

Article Snippet: Primary antibodies used were as follows: Mouse (M) anti-4G2 (Cat# Ab00230-2.0, Absolute Antibody), M anti-DENV NS1 (Type 2/3/4) (Cat# MAB94441-100, R&D Systems), M anti-NXT1 (Cat#67680-1-Ig, Proteintech), M anti-alpha tubulin (Cat#66031-1-Ig, Proteintech), Rabbit (Rb) anti-beta actin (Cat# 026-42210, Li-cor), Rb anti-DENV NS5 (Cat#NBP2-42901, Novus Biologicals), Rb anti-GAPDH (Cat# 5174S, Cell Signaling Technology (CST)), Rb anti-UPF1 (Cat#12040, CST) Rb anti-UPF2 (Cat#11875, CST), Rb anti-ZIKV NS5 (Cat# GTX133312, GeneTex), Rb anti-ZIKV envelope (Cat# GTX133314, GeneTex).

Techniques: RNA Sequencing Assay, Infection, Comparison, Western Blot, Immunocytochemistry, Staining

Journal: bioRxiv

Article Title: Zika and Dengue Viruses Differentially Modulate Host mRNA Processing Factors Defining Its Virulence

doi: 10.1101/2024.10.18.619084

Figure Lengend Snippet: (A) Heatmap of normalized RNA-seq data for mock versus ZIKV-infected SK-N-SH samples at 1-, 3-, and 5-DPI. (B) Western blot analysis of anti-UPF1, anti-UPF2, and anti-alpha tubulin for mock, ZIKV-, and DENV-infected SK-N-SH at 1-, 3-, and 5-DPI and long-term DENV infections. (C) Immunocytochemistry staining of SK-N-SH mock, ZIKV, and DENV at 1- and 5-DPI. Antibodies used were anti-UPF1 (in green), anti-4G2 (anti-flavivirus, in red), and DAPI (in blue).

Article Snippet: Primary antibodies used were as follows: Mouse (M) anti-4G2 (Cat# Ab00230-2.0, Absolute Antibody), M anti-DENV NS1 (Type 2/3/4) (Cat# MAB94441-100, R&D Systems), M anti-NXT1 (Cat#67680-1-Ig, Proteintech), M anti-alpha tubulin (Cat#66031-1-Ig, Proteintech), Rabbit (Rb) anti-beta actin (Cat# 026-42210, Li-cor), Rb anti-DENV NS5 (Cat#NBP2-42901, Novus Biologicals), Rb anti-GAPDH (Cat# 5174S, Cell Signaling Technology (CST)), Rb anti-UPF1 (Cat#12040, CST) Rb anti-UPF2 (Cat#11875, CST), Rb anti-ZIKV NS5 (Cat# GTX133312, GeneTex), Rb anti-ZIKV envelope (Cat# GTX133314, GeneTex).

Techniques: RNA Sequencing Assay, Infection, Western Blot, Immunocytochemistry, Staining

Journal: bioRxiv

Article Title: Zika and Dengue Viruses Differentially Modulate Host mRNA Processing Factors Defining Its Virulence

doi: 10.1101/2024.10.18.619084

Figure Lengend Snippet: (A) Top panel-Immunocytochemistry of Vero and SK-N-SH mock, ZIKV-infected acute samples at days 1, 3 for Vero and days 1, 5 for SK-N-SH. Antibodies used were anti-UPF2 (in green), anti-4G2 (anti-flavivirus, in red), and DAPI (in blue). Bottom panel-Immunocytochemistry of Vero and SK-N-SH mock, DENV-infected acute samples at days 1, 3 for Vero and days 1, 5 for SK-N-SH. Antibodies used were anti-UPF2 (in green), anti-4G2 (anti-flavivirus, in red), and DAPI (in blue). (B) A comparison of normalized RNA-seq reads represented in reads per million for UPF2 and visualized in IGV. Comparisons shown for mock, ZIKV-, and DENV-infected Vero and SK-N-SH samples at 1-, 3-, 5-DPI, and long-term infected samples.

Article Snippet: Primary antibodies used were as follows: Mouse (M) anti-4G2 (Cat# Ab00230-2.0, Absolute Antibody), M anti-DENV NS1 (Type 2/3/4) (Cat# MAB94441-100, R&D Systems), M anti-NXT1 (Cat#67680-1-Ig, Proteintech), M anti-alpha tubulin (Cat#66031-1-Ig, Proteintech), Rabbit (Rb) anti-beta actin (Cat# 026-42210, Li-cor), Rb anti-DENV NS5 (Cat#NBP2-42901, Novus Biologicals), Rb anti-GAPDH (Cat# 5174S, Cell Signaling Technology (CST)), Rb anti-UPF1 (Cat#12040, CST) Rb anti-UPF2 (Cat#11875, CST), Rb anti-ZIKV NS5 (Cat# GTX133312, GeneTex), Rb anti-ZIKV envelope (Cat# GTX133314, GeneTex).

Techniques: Immunocytochemistry, Infection, Comparison, RNA Sequencing Assay

Journal: International Journal of Molecular Sciences

Article Title: Disrupted Post-Transcriptional Regulation of Gene Expression as a Hallmark of Fatty Liver Progression

doi: 10.3390/ijms252011054

Figure Lengend Snippet: ( A ) Western blot analysis detecting Upf2 and β-Actin in the liver of WT or Upf2 Hep-KO mice. ( B ) qPCR detecting the relative expression of genes indicated ( n = 4 for WT and n = 2 for Upf2 Hep-KO ). Data shown as the mean ± SEM, * p < 0.05. ( C ) Western blot analysis detecting phospho-Upf1 (Ser1127) or β-Actin in mice fed with chow or GAN diet for 3 or 6 months. Each lane represents samples pooled from 5 to 7 mice.

Article Snippet: The following primary antibodies were used for immunoblotting: Upf2 (Cell Signaling Technology, Cat# 11875, Danvers, MA, USA), β-Actin (Cell Signaling Cat# 8457), phospho-Upf1 (Ser1127) antibody (MilliporeSigma, 07-1016, Burlington, MA, USA).

Techniques: Western Blot, Expressing